Valencia, Spain | 16 - 18 May 2024 11th EORNA Congress ePoster Guided View 31 ePoster Guided View: ePoster Mini Presentations 348 Failure to sterilize hinged instruments submitted to low temperature sterilization Camila Bruna1, Kazuko Uchikawa Graziano2 1Universidade de São Paulo, São Paulo, Brazil, 2Universidade de São Paulo Background: Although rongeurs and bone cutters are made of stainless steel and therefore autoclavable, not infrequently these instruments are sterilized at low temperature. Objectives Our aim was to determine whether the sterilization of hinged instruments is effective using low-temperature sterilization methods. Methods: An experimental laboratory study was conducted. We submitted 3 rongeurs and 2 bone cutters to three different low temperature sterilization methods, ethylene oxide gas (EO), hydrogen peroxide gas plasma (HP), and lowtemperature steam formaldehyde (LTSF) and to steam sterilization. To analyze the effectiveness of sterilization, we inserted substrate papers of biological indicators indicated to each method (Bacillus atropheus for EO and Geobacillus stearothermophilus for steam sterilization, HP and LTSF) between the faces of the instrument. After sterilization, in the biological safety cabinet and using aseptic technique, the packages of both rongeurs and bone cutters were opened and with the help of sterile forceps the substrate papers were removed and inoculated directly into test sterilized tubes containing Sodium Thioglycolate culture medium (BD Difco™, France) and incubated in an oven at 55°C± 2°C for 7 days. In the absence of microbial growth, samples were heat shocked at 80°C for 20 minutes and re-incubated at 55°C± 2°C for an additional 72 hours for final reading. Results: None of the substrate paper inserted into the instruments that were submitted to steam sterilization showed microbial growth. Four out of 5 substrate paper inserted in instruments sterilized in HP were not effectively sterilized. Conclusion Our results show the risk of ineffective sterilization of articulated materials, which cannot be kept open during sterilization. 422 Investigation of children›s desire for information during the perioperative period Foteini Bakalaki1, Nikolaos Zavras2, Panteleimon Perdikaris3, Maria Polikandrioti4, Evaggelos Dousis4, Aphrodite Zartaloudi4, Ioannis Koutelekos4 1General Children Hospital “Agia Sophia”, Athens, Greece, 2Medical School of EKPA, Athens, Greece, 3University of Peloponnese, Tripoli, Greece, 4University of West Attica, Athens, Greece Background: The perioperative discussion between the child patient and the healthcare providers could be ideal. Both children and their parents need comprehensive information preoperatively. Objectives: The aim of this study is to estimate the desire for information of the child patients preoperatively aged 7-17 years old, as long as to clarify the interaction between the level of anxiety of these children and their need for information during the perioperative period. Methods: The study is cross-sectional observational. The sampling was convenient. We collected 80 questionnaires from children aged between 7-17 years old, both boys and girls who underwent surgical operation. As an instrument of interviews was used the translated in Greek CDI (Children’s Desire for Information) questionnaire with internal consistency coefficient Cronbach’s alpha 0,918. In additional, we used the Greek version of STAIC-State and STAIC-Trait with internal consistency coefficients 0,922 and 0,826 respectively. Results: From the statistical analysis of the data the results obtained highlighted the following factors as important for their interpretation. Thus, the multivariate analysis of the “CDI-total score” indicates that the child’s fear of surgery with p<0.012 is decisive and points out the way children feel about surgery when the information they have received is insufficient and incomplete. The family status of the caregiver with p<0.006 also plays its role as a socio-demographic factor in the degree of desire for information from the child who is going to be operated on. As well as with p<0.016 the child’s anxiety state analyzed from the answers to the Greek version of STAIC-State questionnaire. Conclusion: Therefore, we conclude that the ability of healthcare professionals to adequately understand children’s desire for information preoperatively and their ability to resolve their queries and instill confidence in their patients is paramount as this can alleviate the fear and anxiety that children experience.
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