10 11th EORNA Congress Valencia, Spain | 16 - 18 May 2024 Symposia and innovation programme grant agreement No 101057825. The objectives are to: • Carry out a self-evaluation and compare compliance with the SAFEST Perioperative Patient Safety Standardized Practices across ten hospitals in five countries: Spain, The Netherlands, Portugal, Estonia and Czech-Republic. • Gain insight and raise awareness for patient safety in the entire perioperative care. Methods: First, multi-professional implementation teams were established in each participating hospital. These teams conduct a guided self-evaluation through team review, document review, patient interviews, medical-record review and observation. A total of 154 measurable elements (extracted from 101 recommendations resulting from an extensive Delphi-procedure) are assessed covering a wide range of perioperative safety aspects, e.g. preoperative evaluation and complication prevention. Patient research partners and representatives were actively involved, e.g. in formulating the recommendations and (preparing) the patient interviews. The self-evaluation tool includes an online scoring system, using a 3-point scale ranging from poorly, partially, to fully implemented (0,5,10 points respectively). Finally, the scores between the participating hospitals are compared. Results: The results from the self-evaluation will be presented, covering the variation between hospitals and improvement areas. Experiences with the feasibility of the tool will also be shared. Conclusion: The SAFEST self-evaluation tool provides a broad structured insight into the extent of implementation of the defined safety standards for surgical care. Results are widely supported from a multi-professional point of view, including patient representatives, which can be used for benchmarking, learning and knowledge-sharing. 116 Real-time monitoring of fluorescent bioparticles - an alternative to traditional CFU measurements regarding air quality in operating theatres Lise-Lott Larsson1 1, Johan Nordenadler1, Mattsson Janet1, Gunilla Björling1, Harald Brismar1 1Stockholm, Karolinska Institutet, Stockholm, Sweden Background: Microbiological cleanliness of the air is crucial for infection-sensitive procedures as there is a risk of airborne bacteria-carrying particles contaminating surgical wounds or surgical instruments. The standard method for controlling air cleanliness during surgeries is the measurement of bacterial presence per volume unit of air - Colony Forming Units (CFU/m3). The analysis gives answers no earlier than 5 days after sampling. A new method is real time measurement of fluorescent bioparticles per unit volume of air (FBP/dm3), which could constitute a surrogate measure for the presence of bacteria in the air. Objectives: To validate the measuring instrument Bio Aerosol Monitoring System (BAMS), by comparing simultaneous measurements of FBP/dm3 with CFU/m3. Methods: 15 arthroplasty surgeries were performed in two modern operating theatres with mixed ventilation (approx. 49 air changes per hour). A traditional air sampler (Sartorius MD8 Air Sampler) and a BAMS were placed next to each other about 3 meters from the operating table. Six parallel 10 - minute measurements were performed per surgery. Only particles larger than 3 µm were analysed. The measurement pairs were plotted with FBP/dm3 on the x-axis and CFU/m3 on the y-axis. Simple linear regression was performed and r2 was calculated as a measure of the ratio of FBP/dm3 to CFU/m3. Results: The mean value of FBP/dm3 and CFU/m3 during individual 10-minute measurements showed moderate correlation (r2=0.36, ci 0.04-0.67). The mean value of FBP/dm3 per operation and mean of CFU/m3 per operation showed strong correlation (r2=0.72, ci 0.52-0.92). Conclusion: Analysis of fluorescent bioparticles during surgery is an opportunity to estimate air quality in the operating theatres in real time. The method gives a good estimate of what simultaneous CFU measurement had shown, but has the advantage of being faster, simpler and cheaper to perform. Controlling air cleanliness during infection-sensitive surgeries increases patient safety.
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