864
LBP3 enhanced antitumor immune responses partly via
releasing the brakes of immunosuppression in the tumor
microenvironment of H22 tumor-bearing mice
Deng, X.L.
1,2
, Hu,M.H.
1
,Wang, Y.Y.
1
,Ma, F.L.
1
, Luo, X.
2
, Zhou, L.
2
1
InfinitusChineseHerbalImmunityResearchCentre,Guangzhou,China,
2
GuangzhouUniversityofChineseMedicine, Guangzhou, China
Recent studies have shown that targeting of immunosuppressive
cells and immune checkpoints in the tumor microenvironment
is a promising approach to enhance the efficacy for cancer
immunotherapy. Polysaccharide from Lycium barbarum LBP
inhibits tumor growth in vivo at least partly via improving antitumor
immunity. However, themechanismsof howLBPmounts aneffective
antitumor immune response are not well understood. In the present
study, the modulation of a polysaccharide fraction from LBP (LBP3)
on the immune system inH22 tumor-bearingmicewas investigated.
TheresultsshowedthatLBP3couldeffectivelyinhibit the solid tumor
growth of H22 tumor-bearing mice. Thymus indexes, the numbers/
percentages of T cells in peripheral blood and tumor tissues, killing
activities of natural killer (NK) cells, cytotoxic T lymphocytes (CTL)
andperitonealmacrophages againstH22 cellswere also improved in
three LBP3 treated groups. Besides, LBP3 treatment could decrease
the secretion of TGF-β, IL-6, IL-2 and IL-10 levels in serum. The
percentages of regulatory T cells (Tregs) in tumor-draining lymph
nodes (TDLN) and tumor tissues were also significantly decreases
in the all LBP3 treated groups. Furthermore, LBP3 treatment could
effectively down regulate the PD-1 expression on T cells in TDLN
and tumor tissues. Taken together, these findings indicate that LBP3
enhanced antitumor immune responses partly via releasing the
brakes of immunosuppression in the tumor microenvironment of
H22 tumor-bearing mice.
All the authors contributedequally to thismanuscript).
Interaction between breast cancer cells and microglia in the
microenvironment of brain metastasis
Foo,S.L.
1,2
,Lim,H.K.L.
1,2
1
NUSGraduateSchoolforIntegrativeSciencesandEngineering,
NationalUniversityofSingapore,Singapore,Singapore,
2
Inflammation
andCancerLaboratory,ImmunologyProgramme,YongLooLinSchool
ofMedicine,NationalUniversityof Singapore,Physiology,Singapore,
Singapore
The occurrence of breast cancer metastases is preferential to certain
organs, and one of it being the brain. As the brain microenvironment
is continuously monitored by microglia- a major component of the
brain immune system, invasion of metastatic breast cancer cells to
the brain should stimulate an immune response in the microglial
cells. In this study, we use an in vitro model comprising of microglia
and metastatic breast cancer cell lines to investigate microglial
reactionstometastaticbreastcancercells, particularly inmodulating
tumour cell proliferation, cell invasion, immune suppression, and
microenvironment modification. In contrast to macrophages, co-
cultures and insert-cultures of microglia and breast cancer cells
demonstrated that microglia are activated by tumor cell-oriented
factors, resulting in priming or sensitization of microglia to adopt
an M1 (pro-inflammatory), rather than M2 (anti-inflammatory)
phenotype. It is alsoobserved that there are chemoattractant factors
secretedbybreastcancercells thatinducedasignificantchemotactic
activity for microglial cells in vitro, and the recruited microglia have
further been found to reduce cancer cells growth. The findings of
the metastasis-antagonizing role of microglia suggest that there
are possibly differences between tumour associated peripheral
macrophagesandbrainintrinsicmicrogliaintermsoftheirresponses
towards breast cancer cells and that differences may also exist in
microglial reactions toward primary breast cancer cells as compared
to cancer cells that have metastasized to the brain. Therefore, it
wouldbeof interest to investigate the functional aspectsofmicroglia
in invasion, dormancy, and relapse of brainmetastatic breast cancer.
Correlation betwen the metabolome and binder of ST2
receptor in chronic periodontics in elderly
Borges,A.
1
,Carvalho,M.
2
,Venturini,G.
3
,Vieira,C.
4
,Paulino,T.
5
,Botelho
Miguel,C.
6
,Oliveira,C.
6
,Pereira,A.
3
,Binvignat,O.
7
, Rodrigues,W.
4,7,8
1
FaculdadeMineirense-Fama,Odontology,Mineiros-GO,Brazil,
2
FaculdadeMineirense-FAMA,Odontology,Mineiros,Brazil,
3
Heart
Institute (InCor)/Univ of SaoPauloMed Sch, SãoPaulo, Brazil,
4
Federal
University of Uberlandia, Uberlandia, Brazil,
5
FederalUniversityof
GoiasTrianguloMineiro-CEFORES,Uberaba,Brazil,
6
FederalUniversity
ofTrianguloMineiro,Uberaba, Brazil,
7
FaculdadeMineirense-Fama,
Mineiros-GO,Brazil,
8
FederalUniversityofGoiasTrianguloMineiro,
Uberaba,Brazil
Introduction:
High relationship of senescence with the emergence
and/or compromise of disease in oral cavity is a triggering factor
of disease in this age group including chronic periodontal disease
(CPD). Some markers have been associated with disease prognosis,
as well as systemic diseases, such as ST2 binder.
Objectives:
Evaluate the correlation between metabolites and
ST2 receptor binder in crevicular fluid in the elderly.
Methods:
All
procedures were approved by the Ethics Committee in Research
of the Federal University of Triangulo Mineiro, under number:
017430/2014. Twenty individuals were selected after applying the
inclusion and exclusion criteria. They were divided into 2 groups,
CPD (N= 10 - Clinical evaluation+probing depth higher or equal to 3
mm, and at least presence of
marginal bleeding at a site) without disease - Control (n = 10). To
the assessment of metabolites was performed the metabolome
(triplicate - GC/MS Agilent -7890B GC; 5977A MS). The ELISA was
performed for the detection and quantification of IL-33 (R&D
Systems).ThePrismsoftwarewas used for statistical evaluation.
Results:
Wereidentified969metabolites,ofwhich64wereselectedto
analyze. After analysis 5 metabolic (2,3 dihydroxypropyl icosanoate,
glycerol, serine, 5-aminovaleric acid e putrescine) obtained a ratio
CPD/Control greater than 2. The elevation was accompanied of
increase of the ST2-binder, wherewas found a positive correlation.
Conclusion/discussion:
Thedatapointtoarelationshipofmetabolic
activity inducible IL-33/ST2 inCPD in the elderly.