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along with an remarkably attenuated IRF3 signaling by L-pampo
administration. However, at an effector phase, L-pampo and Pam3
comparably induced expansion of CXCR5
+
PD-1
+
follicular helper
T cells. Interestingly, during the memory phase, L-pampo showed
prominent maintenance of antigen-specific CD4 T cells together
with a high level of antibody titers when comparing to PolyI:C or
pam3 alone. Collectively, thesefindings suggest that combinationof
TLR2 and TLR3 ligands modulates cytokine production and prolong
CD4 T cells that may lead to prominent antibody response and
expansion ofmulti-functional CD4T cells uponboosting.
Adjuvant therapy of chronic uroinfections in colonised
patients
Vaskova,S.
1,2
,Blazickova,S.
2,3
1
LaboratoriaPiestany,Dept.ofClinicalMicrobiology,Piestany,Slovakia,
2
TrnavianUniversity,FacultyofHealthScienceand SocialWork,Trnava,
Slovakia,
3
LaboratoriaPiestany,Dept.ofClinicalImmunology,Piestany,
Slovakia
Introduction:
Urinary tract infections represent themost commonly
acquiredbacterialinfections.Identicalbacterialspecies permanently
isolated from urine of patients with chronic uroinfections are
supposed to colonize distal segment of genitourinary tract and to
form biofilm on mucous surface. Biofilms are the well-organized
microbial communities and decrease the susceptibility to the host
immune system and to the antimicrobial agents. Therefore, therapy
of chronic infections should be different than therapy of acute ones.
Aim:
To compare antibiotic therapy of chronic uroinfections with
adjuvant immunomodulatory therapy in colonised patients.
Material and methods:
36 patients with monobacterial chronic
uroinfections and bacterial species permanently isolated from urine
that produce biofilm
in vitro
. Patients were classed into two groups:
group A - patients treatedwith antibiotics (n = 29), group B - patients
treated with antibiotics and adjuvant immunomodulatory therapy
(n=7). Inflammatory andblood elements parameters were analysed
in thepatients.
Results:
We did not observe statistically significant differences
between clinical benefits in patients with antibiotic and adjuvant
immunomodulatory therapy. Clinical benefit after antibiotic
treatment was observed in 9 patients (31 %); clinical benefit after
adjuvant immunomodulatory therapy was observed in 4 (57 %)
patients. We did not see statistically significant changes in other
laboratory parameters.
Conclusion:
The present study is a pilot study. Adjuvant therapy
seems to have benefit for patients with chronic uroinfections.
We would like to focus on analyse changes in immune system in
colonised patients with chronic uroinfections and in patients with
acute uroinfections.
Keywords:
chronic uroinfections, biofilm, immunomodulatory
therapy
Strategies for the
in silico
selection of immunogenic epitopes
using non-model organisms: use case with
Histoplasma
capsulatum
RubioC.,M.
1,2
,CanoRestrepo,L.E.
1,3
,OchoaDeossa,R.A.
4
1
CorporaciónparaInvestigacionesBiológicas(CIB),MicologíaMédicay
Experimental,Medellín,Colombia,
2
Universidadde Antioquia,Instituto
deBiología,Medellín,Colombia,
3
UniversidaddeAntioquia,Escuelade
Microbiología,Medellín,Colombia,
4
UniversidaddeAntioquia,SIU-
PECET-CIEMTO,Medellín,Colombia
The dimorphic fungal pathogen
Histoplasma capsulatum (Hc)
causes respiratory and systemic diseases. The generation of
a protective immune response to the pathogenic fungus
Hc
is
critically dependent on the interaction between antigen-reactive
T cells and macrophages. Recently, new findings are available
about the proteome and secretome of this fungus. Nevertheless,
there is a huge gap in knowledge regarding interactions between
constituent molecules of the fungus and the host during infection.
Therefore, the evaluation of new immunological interfaces will
serve us to deepen in the pathogenesis of Histoplasmosis, and
then propose possible molecular targets for vaccine or diagnostic
tests. Approaches based on bioinformatics tools have contributed
to know more about the immunogenicity generated by CD8T cells,
optimizing and directing experimental efforts to elucidate the
problem. According to the principles of reverse vaccinology, we
propose a pipeline that involves: i) search for molecular patterns
from immunogenic/antigenic proteins experimentally proven; ii)
find the patterns on the complete set of annotated proteins to find
unique epitopes; iii) identify whether the proteins are secreted
and predict the feasibility of being recognized by CD8T cells.
Beta-lactamase, aryl-alcohol-dehydrogenase and GARP complex
component arepotential newtargets in
Hc
relatedwith the adaptive
immune response. Functionalities such as the union to penicillin,
catalysis of aromatic alcohol and protein transport between Golgi,
endosomal, and vacuolar compartments.are associated to these
targets respectively. The computational protocol was applied and
a set of epitopes predicted for further experimental validation.
Colciencias grant 221356933526.
Regulation of inflammasome during clinical sepsis:
a PCR array study
Esquerdo,K.F.
1
,Shama,N.K.
1
,Brunialti,M.K.C.
1
,Machado,F.R.
2
,Silva,E.
3
,
Rigato,O.
4
,Salomao,R.
1
1
DisciplinadeInfectologia,EscolaPaulistadeMedicina,HospitalSão
Paulo,UniversidadeFederaldeSãoPaulo-UNIFESP, Medicine, Sao
Paulo, Brazil,
2
DisciplinadeAnestesiologia, EscolaPaulistadeMedicina,
Hospital SãoPaulo, Universidade FederaldeSãoPaulo-UNIFESP,
Medicine,SaoPaulo,Brazil,
3
HospitalIsraelitaAlbertEinstein,Sao
Paulo,Brazil,
4
Hospital SírioLibanês,SaoPaulo,Brazil
Sepsis is a systemic inflammatory response triggered by an
infection. Nod like receptor family (NLR) is involved in this process
through inflammasome oligomerization. To study the regulation of
inflammasome under sepsis, we analyzed patients (survival n=19,
non-survival n=8) at admission and after 7days of follow-up (survival
n=13, non-survival n=5) with healthy volunteers (n=11). In brief,
mononuclear cells were separated, cDNA synthesized from isolated
RNA fromcells and analyzed by real time PCR array (35 genes). Genes
were considered differentially modulated when the expression
was higher than ±1.5 and p≤0.05. The resulted data was processed
through Ingenuity Pathway Analysis for functional analysis and
interactions. The gene expression study enables us to identify
alteration in 8 genes at admission and 7 genes after day 7 in survival,
16 genes at admission and 11 genes after day 7 in non-survival.